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101.
102.
Manjunatha S. Muttigi Byoung Ju Kim Bogyu Choi Inbo Han Hansoo Park Soo-Hong Lee 《International journal of molecular sciences》2020,21(23)
Adipose-derived mesenchymal stromal cells (Ad-MSCs) are a promising tool for articular cartilage repair and regeneration. However, the terminal hypertrophic differentiation of Ad-MSC-derived cartilage is a critical barrier during hyaline cartilage regeneration. In this study, we investigated the role of matrilin-3 in preventing Ad-MSC-derived chondrocyte hypertrophy in vitro and in an osteoarthritis (OA) destabilization of the medial meniscus (DMM) model. Methacrylated hyaluron (MAHA) (1%) was used to encapsulate and make scaffolds containing Ad-MSCs and matrilin-3. Subsequently, the encapsulated cells in the scaffolds were differentiated in chondrogenic medium (TGF-β, 1–14 days) and thyroid hormone hypertrophic medium (T3, 15–28 days). The presence of matrilin-3 with Ad-MSCs in the MAHA scaffold significantly increased the chondrogenic marker and decreased the hypertrophy marker mRNA and protein expression. Furthermore, matrilin-3 significantly modified the expression of TGF-β2, BMP-2, and BMP-4. Next, we prepared the OA model and transplanted Ad-MSCs primed with matrilin-3, either as a single-cell suspension or in spheroid form. Safranin-O staining and the OA score suggested that the regenerated cartilage morphology in the matrilin-3-primed Ad-MSC spheroids was similar to the positive control. Furthermore, matrilin-3-primed Ad-MSC spheroids prevented subchondral bone sclerosis in the mouse model. Here, we show that matrilin-3 plays a major role in modulating Ad-MSCs’ therapeutic effect on cartilage regeneration and hypertrophy suppression. 相似文献
103.
基于太赫兹光谱的人参和西洋参鉴别 总被引:1,自引:0,他引:1
为了快速区分人参和西洋参,依据两者所含的人参皂苷在太赫兹波段具有不同指纹光谱,提出了一种基于人参皂苷的太赫兹光谱鉴别人参和西洋参的方法。采用MATLAB软件识别人参和西洋参的太赫兹光谱,同时根据太赫兹光谱特征并通过标准的主成分分析(PCA)区分出人参、西洋参。实验结果表明,基于太赫兹光谱的主成分分析可精确区分出人参、西洋参,并且还可以应用于其他类似物质的区分中。 相似文献
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The discrete element method (DEM) has been extensively adopted to investigate many complex geotechnical related problems due to its capability to incorporate the discontinuous nature of granular materials. In particular, when simulating large deformations or distortion of soil (e.g. cavity expansion), DEM can be very effective as other numerical solutions may experience convergence problems. Cavity expansion theory has widespread applications in geotechnical engineering, particularly to the problems concerning in situ testing, pile installation and so forth. In addition, the behaviour of geomaterials in a macro-level is utterly determined by microscopic properties, highlighting the importance of contact models. Despite the fact that there are numerous contact models proposed to mimic the realistic behaviour of granular materials, there are lack of studies on the effects of these contact models on the soil response. Hence, in this study, a series of three-dimensional numerical simulations with different contact constitutive models was conducted to simulate the response of sandy soils during cylindrical cavity expansion. In this numerical investigation, three contact models, i.e. linear contact model, rolling resistance contact model, and Hertz contact model, are considered. It should be noted that the former two models are linear based models, providing linearly elastic and frictional plasticity behaviours, whereas the latter one consists of nonlinear formulation based on an approximation of the theory of Mindlin and Deresiewicz. To examine the effects of these contact models, several cylindrical cavities were created and expanded gradually from an initial radius of 0.055 m to a final radius of 0.1 m. The numerical predictions confirm that the calibrated contact models produced similar results regarding the variations of cavity pressure, radial stress, deviatoric stress, volumetric strain, as well as the soil radial displacement. However, the linear contact model may result in inaccurate predictions when highly angular soil particles are involved. In addition, considering the excessive soil displacement induced by the pile installation (i.e. cavity expansion), a minimum distance of 11a (a is the cavity radius) is recommend for practicing engineers to avoid the potential damages to the existing piles and adjacent structures. 相似文献
108.
基于深度特征的目标检测方法Faster R-CNN在火焰检测任务上存在检测效率低的问题,因此提出了基于颜色引导的抛锚策略。该策略设计火焰颜色模型来限制锚的生成,即利用火焰颜色约束锚的生成区域,从而减少了初始锚的数量,提升了计算效率。为了进一步提高网络的计算效率,将区域生成网络中的卷积层替换成掩膜卷积。为了验证所提方法的检测效果,采用BoWFire和Corsician数据集进行验证。实验结果表明,该方法实际检测速度相较于原Faster R-CNN提高了10.1%,BoWFire上该方法的火焰检测F值为0.87,Corsician上该方法的准确度可达99.33%。所提方法可以提高火焰检测的效率,并能够准确检测图像中的火焰。 相似文献
109.
Qi Li Xing Wang Fuan Wang Da Chen Xiaolei Xiao Helin Zou 《Ceramics International》2018,44(7):7709-7715
Pb(NdxZr0.52Ti0.48)O3 (PNZT) (x = 0%, 1%, 2%, 3%, 4%, 5%) thin films were prepared by sol-gel process to investigate the effects of neodymium substitution on crystalline orientation, microstructure and electric properties of lead zirconate titanate (PZT) films. X-ray diffraction (XRD) and scanning electron microscope (SEM) analysis showed that PNZT films with Nd doping concentration below 3% exhibited dense perovskite structure with (100) preferred orientation. The average grain size of PNZT films decreased as the Nd substitution increased. The maximum dielectric constant, remnant polarization and minimum coercive field were obtained in 2% Nd-doped PZT films. Fatigue resistance was also improved significantly with 2% Nd dopant. 相似文献
110.
Phase change materials (PCM) have an increasingly more important role as a thermal energy storage (TES) media. However, leakage problem of PCM causes limitation during their integration in TES systems. Therefore, the encapsulation of PCMs is attracting research interest to extend usage of PCMs in real TES applications in recent years. In this study, hydroxystearic acid (HSA) was encapsulated with polymethyl methacrylate (PMMA) and different PMMA comonomer shells via emulsion polymerization method for the first time in literature. HSA with high melting temperature range (74–78°C) can widen the scope of using PCMs, and the encapsulated form can make it more versatile. The chemical structures, morphologies, and thermophysical properties of capsules were determined by FT‐IR, SEM, DSC, TGA, and thermal infrared camera. Among the produced HSA capsule candidates, PMMA‐HEMA is the most promising with latent heat of 48.5 J/g with melting range of 47 to 85°C. SEM analysis indicated that the capsules have spherical shape with compact surface at nano‐micro (100–440 nm) size range; however, some capsules exhibited agglomeration. 相似文献